The miniDAWN® TREOS® II determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.

The TREOS II not only offers superb sensitivity—it further enhances productivity thanks to some unique capabilities: field serviceability, upgradeability to UHPLC and One-Click MwTM.

Included with each miniDAWN is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the TREOS II incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.

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Molar Mass Range 200 Da to 10 MDa1
Molecular Size Range (rg) 10 nm to 50 nm1, up to 150 nm with shape-specific models
Molecular Size Range (Rh, with WyattQELS DLS module or DynaProNanoStar) Depends on the scattering angle:
In flow mode, 0.5 nm – 50 nm1,2
batch mode, 0.5 nm – 1µm1
Sensitivity 25 ng of 100 kDa polystyrene in THF3 500 ng of BSA in PBS4
Mobile Phase Compatibility All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica, and Kalrez.
MALS Detectors High-gain, high dynamic range, static light scattering detectors at 3 angles.
Auxiliary Detectors Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.
A/D Resolution Detector dynamic range > 16,000,000:1
DLS Detector (optional) WyattQELS dynamic light scattering module installs directly inside the miniDAWN chassis.

Alternatively, the optical fiber pickup of the DynaProNanoStar cuvette-based DLS instrument may be installed in the miniDAWN.
Laser Properties
Laser Wavelength 658 nm (nominal). Each instrument is individually characterized.
Laser Power Control Programmable 10% - 100%
Flow Cells Fused Silica, optimal for solvent refractive index < 1.50
Analog Inputs 2 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V
Analog Outputs 1 analog output from user selectable measurements channels -10 V to +10 V
Other Inputs/Outputs Alarm In, Alarm retransmit, Auto-Inject in, Autoinject contact closure, Recycle In, Recycle valve power
Computer Interface Ethernet
Processed Data Transmission Rate 36.6 Hz to 0.2 Hz
Front Panel Display 162.5 mm, 16-bit, high resolution touch screen
1Typical. Actual range depends on dn/dc, sample concentration, sample conformation and chromatography conditions.

2Upper limit assumes a flow rate of 0.5 mL/min.

3Under HPLC-SEC conditions with 7.8x300 mm GPC columns.

4Under HPLC-SEC conditions with Wyatt 7.8x300 mm SEC protein columns.

Host PC requirements may be found in Computer Requirements.

Specifications subject to change without notice.