MP-SPR Navi™ 220A NAALI
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Features
- Automation of samples from 96 and 384 well plates allows for unattended runs
- Software switch for serial and parallel injections
- Partial loop injections providing minimal sample consumption
- Simultaneous measurements in 2 fluidic channels
- Two independent wavelength measurements in each fluidic channel, enabling detection of thickness and refractive index or conformational changes
- Instrument can be equipped optically with up to 4 different wavelenghts measured from each liquid channel for optimal data for layer characterization
- Exceptionally wide angular range 40˚ – 78˚ enables measurements of small organic molecules and large proteins, but also metallic nanoparticles and cells. Most importantly, it enables in-line bulk effect separation with PureKinetics™
- Measurements of kinetic constants, concentration and competition analysis, affinity and EC50
- Controlled buffer flow conditions with precise syringe pumps and integrated degasser
- Software package for convenient control of the instrument and easy analysis of the results. The instrument comes with TraceDrawer™ for MP-SPR Navi™ – a powerful software for the determination of affinity and kinetic constants and more. MP-SPR Navi™ LayerSolver for the determination of thickness and RI from multiple wavelength measurements is an optional module
- MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and remove for further analysis with other methods such as AFM or microscopy
- Complement with electrochemistry, high chemical resistant and gas flow cells as well as fluorescence setup provides combined or validation measurements
| Number of fluidic channels | 2 |
| Autosampler for liquids | 96 and 384 well plate,6-vials |
| Wavelengths in each measurement channels | Up to 4 |
| Unattended run | Yes |
| Buffer degasser | Yes |
| Precise syringe pumps | Yes |
| Temperature control | 15-45°C |
| Compatibility with organic solvents | Optional |
(Protein concentration analysis from cow milk using MP-SPR NaviTM 220A NAALI)
Flow-cells
We provide a range of application-specific, user-replaceable flow cells. Standard materials include PDMS, PEEK, and Kalrez, tailored for kinetic or material characterization. Specialized cells combine MP-SPR with electrochemistry, fluorescence, or live-cell studies. All support air/liquid, with a gas cell for pressurized gas.
MP-SPR Navi™ Immobilizer
Our MP-SPR Navi™ Immobilizer is a specialized stand-alone holder for chemical modification and functionalization of sensor surfaces, compatible with MP-SPR Navi™ sensor slides and slide holders.
More information in Application Note #130 >>>
Additional wavelengths
Simultaneous multi-wavelength SPR measurements, using two to four wavelengths, provide accurate thickness and refractive index data. Three or four wavelengths remove the need for additional assumptions. This technique is vital for material characterization, vesicle analysis, and live cell studies. Navi™ instruments support two wavelengths, while the OTSO, VASA, and NAALI models offer four.

MP-SPR sensor slides
BioNavis provides high-quality MP-SPR sensor slides, compatible with all Navi™ instruments, for precise measurements. Our diverse range caters to various applications. The user-friendly slide holder simplifies ex situ functionalization via spin coating, Lb, self-assembly, CVD, or ALD.
Browse BioNavis for sensor surfaces matching your needs: https://bionavis.com/products/sensor-slides
