DAWN®
The DAWN® is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. The DAWN® offers the highest sensitivity, the widest range of molecular weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.
The DAWN® is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.
Included with each DAWN® is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.
The DAWN® is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.
Included with each DAWN® is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.
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SPECIFICATIONS
SPECIFICATIONS
| Molar Mass Range | 200 Da to 10 MDa1 |
| Molecular Size Range (rg) | ≈ 10 to 500 nm1, up to 1000 nm with shape-specific models |
| Molecular Size Range (Rh, with WyattQELS DLS module or DynaProNanoStar) | Depends on the scattering angle: In flow mode, 0.5 nm – 300 nm1,2 batch mode, 0.5 nm – 1µm1 |
| Sensitivity | 10 ng of 100 kDa polystyrene in THF3 200 ng of BSA in PBS4 |
| Fluidics | |
| Mobile Phase Compatibility | All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica, and Kalrez. |
| Optics | |
| Detectors | |
| MALS Detectors | High-gain, high dynamic range, static light scattering detectors at 18 angles. |
| Auxiliary Detectors | Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality. |
| A/D Resolution | 24 bit (detector dynamic range > 16,000,000) |
| DLS Detector (optional) | WyattQELS dynamic light scattering module installs directly inside the miniDAWN® chassis.
Alternatively, the optical fiber pickup of the DynaProNanoStar cuvette-based DLS instrument may be installed in the miniDAWN®. |
| Laser Properties | |
| Laser Wavelength | 658 nm; 785 nm available for fluorescence reduction (with lower sensitivity than 658 nm) |
| Laser Power Control | Programmable 10% – 100% |
| Flow Cells | Fused Silica, optimal for solvent refractive index less than 1.50 F2, optimal for solvent refractive index above 1.50 |
| Electronics | |
| Analog Inputs | 4 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V |
| Analog Outputs | 2 analog output from user selectable measurements channels -10 V to +10 V |
| Other Inputs/Outputs | Alarm In, Alarm retransmit, Auto-Inject in, Autoinject contact closure, Recycle In, Recycle valve power |
| Computer Interface | Ethernet |
| Processed Data Transmission Rate | 36.6 to 0.0001 Hz |
| Front Panel Display | 162.5 mm, 16-bit, high resolution touch screen displays signal graphs, instrument settings and diagnostics |
2Upper limit assumes a flow rate of 0.3 mL/min and DLS detection at 160°.
3Under HPLC-SEC conditions with 7.8×300 mm GPC columns.
4Under HPLC-SEC conditions with Wyatt 7.8×300 mm SEC protein columns.
Host PC requirements may be found in Computer Requirements.
STATIC LIGHT SCATTERING
STATIC LIGHT SCATTERING