miniDAWN®
The miniDAWN® determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.
The miniDAWN® not only offers superb sensitivity—it further enhances productivity thanks to some unique capabilities: field serviceability, upgradeability to UHPLC and One-Click Mw™.
Included with each miniDAWN® is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN® incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.
The miniDAWN® not only offers superb sensitivity—it further enhances productivity thanks to some unique capabilities: field serviceability, upgradeability to UHPLC and One-Click Mw™.
Included with each miniDAWN® is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN® incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.
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SPECIFICATIONS
SPECIFICATIONS
| Molar Mass Range | 200 Da to 10 MDa1 |
| Molecular Size Range (rg) | 10 nm to 50 nm1, up to 150 nm with shape-specific models |
| Molecular Size Range (Rh, with WyattQELS DLS module or DynaProNanoStar) | Depends on the scattering angle: In flow mode, 0.5 nm – 50 nm1,2 batch mode, 0.5 nm – 1µm1 |
| Sensitivity | 25 ng of 100 kDa polystyrene in THF3 500 ng of BSA in PBS4 |
| Fluidics | |
| Mobile Phase Compatibility | All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica, and Kalrez. |
| Optics | |
| Detectors | |
| MALS Detectors | High-gain, high dynamic range, static light scattering detectors at 3 angles. |
| Auxiliary Detectors | Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality. |
| A/D Resolution | Detector dynamic range > 16,000,000:1 |
| DLS Detector (optional) | WyattQELS dynamic light scattering module installs directly inside the miniDAWN® chassis.
Alternatively, the optical fiber pickup of the DynaProNanoStar cuvette-based DLS instrument may be installed in the miniDAWN®. |
| Laser Properties | |
| Laser Wavelength | 658 nm (nominal). Each instrument is individually characterized. |
| Laser Power Control | Programmable 10% – 100% |
| Flow Cells | Fused Silica, optimal for solvent refractive index < 1.50 |
| Electronics | |
| Analog Inputs | 2 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V |
| Analog Outputs | 1 analog output from user selectable measurements channels -10 V to +10 V |
| Other Inputs/Outputs | Alarm In, Alarm retransmit, Auto-Inject in, Autoinject contact closure, Recycle In, Recycle valve power |
| Computer Interface | Ethernet |
| Processed Data Transmission Rate | 36.6 Hz to 0.2 Hz |
| Front Panel Display | 162.5 mm, 16-bit, high resolution touch screen |
2Upper limit assumes a flow rate of 0.5 mL/min.
3Under HPLC-SEC conditions with 7.8×300 mm GPC columns.
4Under HPLC-SEC conditions with Wyatt 7.8×300 mm SEC protein columns.
Host PC requirements may be found in Computer Requirements.
Specifications subject to change without notice.
STATIC LIGHT SCATTERING
STATIC LIGHT SCATTERING