The Calypso® II, in conjunction with a DAWN® or miniDAWN® MALS detector, measures binding affinities and absolute, molecular stoichiometries of complex biomolecular interactions.
In addition to equilibrium association constants Kd and absolute stoichiometry of reversible self- and hetero-associations, it can also help determine reaction or aggregation rates, non-specific interaction parameters (virial coefficients) and even automate measurements of weight-average molar mass and dn/dc for polymers.
Included with each Calypso is a copy of CALYPSO software, the most versatile software package available for analysis of biomolecular interactions in solution by light scattering.
Flexibility to re-organize fluid paths and utilize different wetted materials as needed.
Convenient loading of sample and diluent solutions via conical tubes - 5, 15 or 50 mL.
Autoinject Out contact closure signal, to trigger data acquisition by ASTRA or DYNAMICS software for data acquisition and analyses not supported by the CALYPSO software (e.g., DLS acquisition).
Built-in Wash port, allowing the pumps to draw on reservoirs of wash solutions for post-experimental clean-up. Automation of up to two sequential wash solutions, e.g. buffer (to clean out proteins) followed by water (to clean out buffer salts) or detergent followed by water, is possible when a COMET solvent selector valve is added to the setup. These operations are appended to the Method for unattended operation.
ENQUIRY FORM
