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Eclipse® AF4


The Eclipse® AF4 supports all AF4 techniques except Hollow-Fiber Flow Field-Flow Fractionation (HF5), which is covered by the Eclipse DualTec. However, the Eclipse AF4 supports the fritless AF4 channel which prevents the difficulties encountered by certain samples prone to aggregation at the relatively high concentrations typical of the AF4 focusing step. It also supports the semi-preparative channel.

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Eclipse® AF4

  • Robust separation of macromolecules and nanoparticles from 1 nm - 1000 nm with excellent resolution
  • No stationary phase - open channel separation with minimal shear and surface interactions
  • Versatile choice of separation channels, permitting sample loads from µL to mL, including semi-preparative FFF
  • Optimize separation methods to enhance resolution or size range simply by adjusting flow ratios, even programmatically during a run
  • Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ICP-MS, ELISA, etc.)
  • Typical analysis times 10 - 30 minutes
  • Utilize the fritless column for aggregation-prone samples
  • Make use of the highest quality HPLC pumps and autosamplers from Agilent, Shimadzu, Thermo, or Waters
  • Integrate with your favorite HPLC software from Agilent, Dionex or Shimadzu
  • Couple to ICP-MS for elemental analysis of nanoparticles per size bin

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