Electrophoretic Mobility

Möbiuζ®


Wyatt Technology's Möbiuζ® is the first and only light scattering instrument that makes reliable, reproducible, and non-destructiveelectrophoretic mobility measurements of macromolecules as small as 2 nm in radius (as well as micron-size nanoparticles) in most aqueous buffers.

The Möbiuζ characterizes the mobility of proteins and other biomolecules non-destructively, thanks to an innovative design that incorporates 31 parallel detectors denoted as 'massively parallel phase analysis light scattering', or MP-PALS.

MP-PALS is a true enabler for maximum sensitivity and short acquisition times. The combination of a powerful solid-state laser, precision optics and real parallel-plate electrodes near the optical path that do not distort electric field lines, permits application of low voltages with low current. Low voltage and current minimize perturbation of the sample, even in conductive solvents such as physiological saline, since exposure to high voltage may denature the sample and high currents produce heating and convection of the solvent, degrading the measurement.

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The Möbiuζ offers a unique suite of capabilities for mobility and size measurements of proteins, polymers and nanoparticles:

  • Non-destructive analysis of fragile biomolecules
  • Repeatable and accurate measurements in conductive buffers such as physiological saline
  • Simultaneous measurements of size and mobility
  • Automated sample handling or manual measurements
  • Fluorescence blocking filter for sizing of fluorescing nanoparticles such as quantum dots
The Möbiuζ characterizes the mobility of proteins and other biomolecules non-destructively and robustly, thanks to an innovative design enabling some unprecedented capabilities described more fully in the Advanced Technology tab.

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